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Project at a Glance

Contents on the CD Rom

  • Antigens are macromolecule that stimulates an immune response in the body. The word originated from the notion that they can stimulate antibody generation. An antigen may be a foreign substance from the environment such as chemicals, bacteria, viruses, or pollen. An antigen may also be formed within the body, as with bacterial toxins or tissue cells.
  • Antigens can be proteins, polysaccharides and conjugates of lipids with proteins(lipoproteins) and polysaccharides(glycolipids). Antigens can be classified in order of their origins. They are exogenous antigens, endogenus antigens, autoantigens.
  • Exogenous antigens are antigens that have entered the body from the outside, for example by inhalation, ingestion, or injection.
  • Endogenous antigens are antigens that have been generated within the cell, as a result of normal cell metabolism, or because of viral or intracellular bacterial infection.
  • An autoantigen is usually a normal protein or complex of proteins (and sometimes DNA or RNA) that is recognized by the immune system of patients suffering from a specific autoimmune disease.
  • Tumor antigens are those antigens that are presented by the MHC I molecules on the surface of tumor cells. These antigens can sometimes be presented only by tumor cells and never by the normal ones. In this case, they are called tumor-specific antigens (TSAs) and typically result from a tumor specific mutation.
  • The amount of antigen needed to generate a monoclonal antibody varies greatly from one antigen to another. Projects involving antigens that are extremely immunogenic (such as insect lectins) may only require 0.3-0.6 mgs. Projects where the antigen is weakly immunogenic (such as short peptides) may require 1.0 - 5.0 mgs.
  • Strategic Diagnostics Inc. (SDI), a major developer and producer of antibodies and immunoreagents for a broad range of applications, has responded to this market demand by developing Genomic Antibodies, a quicker, cost-effective and more reliable way of producing custom antibodies. With SDI’s Genomic Antibodies technology...physically obtaining the antigen gene; the expression and purification of the antigen; synthesis of peptides; testing, storage, and shipping of the antigen; antigen conjugation; and considerable costs are all eliminated.
  • In response to market demands for an alternative method, SDI has developed an antigen expression method that removes antigen development and production work from the researcher’s laboratory. An additional benefit to moving antigen development and production to SDI is the consistency and reliability of a highly robust system.
  • Novartis announced that it has received a contract from the US Department of Health and Human Services (HHS) of approximately USD 55 million. The contract supports the company's efforts to bring the antigen-sparing capabilities of the MF59 adjuvant to the United States.
  • Retinal S-antigen is a soluble protein of about 50,000 daltons, which is associated with photoreceptor outer segments and which has a potent autoantigenic activity.
  • S-antigen for induction of EAU generally has been prepared from bovine retinas by a procedure involving ammonium sulfate precipitation and gel filtration followed by ion exchange chromatography1 or isoelectric focusing.
  • Peptides are complex molecules and each peptide sequence is unique with regard to its chemical and physical properties. Peptides are synthesized by the solid-phase method using Fmoc chemistry on an Omega 396 multiple peptide synthesizer from Advanced ChemTech.The peptide synthesis service provides a complete spectrum of services that include, synthesis, cleavage, HPLC purification, peptide modifications, cyclization and mass analysis for quality assurance.
General Introduction
  • Antigen, Antibody, Vaccine
  • The Excretion of Antigen
  • Occurrence of a Common Lipopolysaccharide Antigen
  • Antigen
  • Custom Antibody Development
  • Frequently Asked Questions
  • Antigen Retrieval

Technology

  • Better Antibody Detection Through Single Antigen Technology
  • Innovative Solutions in Proteomics
  • Polymer Microstructures for Multianalyte Protein Assay
  • New Antigen Microarray Technology Opens Window to Better Disease Screening
  • A Novel Antigen Technology
  • Use of In Vivo-Induced Antigen Technology
  • Advances in Monoclonal Antibody Technology

Products

  • R antigen Antibodies
  • Blood Group Kell Antigen Antibody [BRIC 203] (ab11463) Datasheet
  • Antigen-Antibody Pens
  • Anti - Onconeural Antibodies
  • Rotavirus Group Specific Antigen Antibody

Antigen Production

  • Standardization of Procedures of Plasmodium Falciparum Antigen Preparation for Serologic Tests
  • Extraction of Equine Infectious Anemia Immunodiffusion Antigen
  • Antigenic Determinant of the Lancefield Group H Antigen of Streptococcus sanguis
  • Monoclonal Antibody Production
  • Polyclonal Antibody Production
  • A general method for antigen production
  • Modulation of Antigen Processing/Presentation Pathways
  • Production of a monoclonal antibody
  • Novel Antigen Production
  • Antibody Polyclonal Production
  • The Antigen Processing Machinery of Class I Human Leukocyte Antigens
  • Reduced Antigen Production
  • Refinement of Polyclonal Antibody Production

Antigen Expression

  • Cluster Analysis of Biological Data - Gene Expression and Antigen Reactivity
  • Identification and Expression of Antigen Genes
  • Mast Cell Regulation of Inflammation and Gene  Expression during Antigen-Induced Bladder Inflammation in Mice
  •  Expression of Polyomavirus Middle T Antigen in Rat Fibroblasts
  • Regulation of Human Monocyte HLA-DR and CD4 Antigen Expression
  • Systematic Variables Affecting Simian Virus 40-Induced TAntigen Expression
  • Targeting Transgene Expression to Antigen-Presenting cells

Antigen Conjugation and Peptide Synthesis

  • Monoclonal Antibody against a Conjugation-Specific Nuclear Antigen in Paramecium Caudatum
  • Conjugation of a self-antigen
  • A Single Antigen-Specific B cell Can Conjugate to Either a Type 1 or a Type 2 helper T cell
  • Peptide Synthesis
  • Synthetic Peptide Vaccine Design

Antigen Purification

  • Characterization and Purification of Lupus Antigen La, an RNA Binding Protein
  • Fraction 1 Capsular Antigen (F1) Purification
  • Purification and Characterization of the Serotype c Antigen
  • Purification of Biologically Active Simian Virus 40 Small Tumor Antigen
  • Purification of Retinal 5-Antigen

Antigen Recognition and Shipping

  • The Interaction of Antibodies With Antigens
  • Antigen Presentation
  • Antigen Receptor Diversity
  • Histocompatibility Molecules
  • Antigen Receptors
  • Technical Suggestions for Sending Antigen
  • Shipping Instructions for Antigens

Antigen Testing

  • Testing for p24 Antigen
  • Carcinoembryonic antigen test
  • Cryptococcus
  • Histoplasma antigen assay-testing at laboratories other than MiraVista Diagnostics
  • Cryptococcal Antigen Test Revisited
Patent
  • Capsular Polysaccharide Adhesion Antigen Preparation, Purification and Use
  • Microorganism Antigen Extraction Methods
  • Antibody Against a Mast Cell Surface Antigen
  • Method and Device of Rapid Antigen Extraction
  • Pertussis Toxin Gene:Cloning and Expression of Protective Antigen
  • Prostate - Specific Membrane Antigen
  • Surface Antigen
  • Nucleotidic Sequence Coding the Surface Antigen of the Hepatitis B Virus, Vector Containing Said Nucleotidic Sequence, Process Allowing the Obtention Thereof And Antigen Obtained ThereBy
Market
  • Keeping Up with the Push to Get to Market Faster
  • Monoclonal Antibodies 2007
  • An Analysis Of Market
  • Antibodies 2005
  • Antigen Production Unit
  • Monoclonal antibodies market
  • Worldwide antibody market to reach $26 billion

Company Profile

  • Biomedica
  • Pevion Biotech
  • AspenBio Pharma, Inc
  • Generex Biotechnology Corporation
  • ImmunoGen
  • Micromum
  • One Lambda
  • Oragenics

Machinery Suppliers

  • Autoclave Trade Leads
  • Infant Incubator
  • Medical Infant Incubator
  • Silicaware
  • Machinery Suppliers

Consultants

  • AYMERIC de PARSEVAL
  • M-SCAN
  • Dorothee Herlyn
  • Experts
  • Immunochemistry Technologies

Project

  • Antigen
  • Activating receptors on antigen presenting cells
  • Production of Fully Human Polyclonal Antibodies in Genetically Modified Pigs
  • Recombinat anti-H5N1 polyclonal antibody for treatment or prophylaxis of avian influenza
  • Antigen Discovery
  • Sea lice of Salmonids
  • Evolution of the antigen presentation system and pathogenic evasion
  • Tumor Antigen Project

Reports

  • Novartis receives US government contract to further develop a novel antigen technology
  • GSK reports significant advance in H5N1 pandemic flu vaccine programme
  • Hepatitis B Surface Antigen Assays
  • Medicare Antigen Preparation
  • OPTN/UNOS Pancreas Transplantation Committee Descriptive Data Request
  • Immunoaffinity Purification of S-Antigen Using Monoclonal Antibodies to Different Antigenic Sites
  • Regulation of squamous cell carcinoma antigen production
  • New Antibody Production Technology Eliminates Need for Antigen Development

Guidelines

  • Hepatitis B - a patient's guide
  • Prostae Specific Antigen (PSA) Screening - a patient's guide
  • Antigen User Guide
  • Polyclonal Antibody Production in Rabbits
  • Polyclonal Antibody Production
  • Guidelines on Polyclonal Antibody Production

Applications

  • Application of Antigen Retrieval
  • Applications of Blood Group Antigen Expression Systems
  • An Application to Autoimmune Diseases
  • Development and Applications of a Bovine Coronavirus Antigen Detection Enzyme
  • Kampo Medicines for Mite Antigen-Induced Allergic Dermatitis in NC/Nga Mice
  • Preclinical Application of Radioimmunoguided
    Surgery Using Anti-Carcinoembryonic Antigen
  • Surfactant Sodium Lauryl Sulfate Enhances Skin Vaccination
  • Using the TAP Component of the Antigen-Processing Machinery as a Molecular Adjuvant

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