- A culture medium is any
material prepared for the growth of bacteria in a laboratory. Microbes
that grow and multiply in or on a culture medium are known as a culture.
Agar is a common solidifying agent for a culture medium.
- Agar is a gel at room
temperature, remaining firm at temperature as high as 65°C. Agar melts
at approximately 85°C, a different temperature from that at which it
solidifies, 32-40°C. This property is known as hysteresis. Agar is
generally resistant to shear forces; however, different agars may have
different gel strengths or degrees of stiffness.
- Agar is typically used
in a final concentration of 1-2% for solidifying culture media. Smaller
quantities (0.05-0.5%) are used in media for motility studies (0.5% w/v)
and for growth of anaerobes (0.1%) and microaerophiles.
- Specifications for
bacteriological grade agar include good clarity, controlled gelation
temperature, controlled melting temperature, good diffusion
characteristics, absence of toxic bacterial inhibitors and relative
absence of metabolically useful minerals and compounds.
- Blood agar plate (BAP)
Contains mammalian blood (usually sheep or horse), typically at a
concentration of 5–10%. BAP are an enriched, differential media used to
isolate fastidious organisms and detect hemolytic activity. β-hemolytic
activity will show complete lysis of red blood cells surrounding colony,
while α-hemolysis will only partially lyse hemoglobin and will appear
green. γ-hemolysis (or non-hemolytic) is the term referring to a lack of
hemolytic activity.
- Blood agar is used to
support the growth of fastidious organisms and to determine the type of
hemolysis (destruction of red blood cell walls) an organism
produces.
- CDC Anaerobic Blood
Agar is an enriched medium for the growth and partial identification of
obligate anaerobes. It also supports good growth of aerobic, facultative
anaerobic and microaerophilic organisms found in clinical materials if
incubated under the appropriate conditions.
- Tryptose Agar is used
for cultivating a wide variety of fastidious microorganisms,
particularly for isolating Brucella. Tryptose Agar with 5% bovine serum,
with or without antibiotics, remains a standard plating medium for the
isolation of brucellae. For isolation of Brucella stains from
contaminated milk, crystal violet (gentian violet) can be added to
Tryptose Agar to suppress gram-positive organisms. Tryptose media can be
supplemented with thiamine or citrate for the cultivation and
maintenance of fastidious aerobic and facultative microorganisms.
- Coagulase Mannitol Agar
is used for the differentiation of Staphylococcus aureus from other
species based on coagulase production and mannitol utilization.
Coagulase Mannitol Agar aids in the differentiation of staphylococci by
indicating the presence of coagulase and the utilization of mannitol.
Coagulase production is dependant on the presence of mannitol, a protein
factor in the brain heart infusion and blood serum (plasma). During
utilization of the mannitol, the pH of the medium drops, causing the
bromcresol purple indicator to change from purple to yellow and
producing yellow zones around these colonies. An opaque area of
coagulated plasma forms around the colonies of organisms that also
produce coagulase.
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General
Information
- Agar and gram
Staining
- Agar plate
- Blood agar
- Culture
Media
- Blood Agar Plates
and Hemolysis
Company
Profile
- German Company
- Company from USA
- Company from Suffolk
- US Company
- Company from
Spain
- Company from
California
- Company from UK
- Indian Company
- Scientific Company
Providing Quality Laboratory Products
Preparation &
Process
- All About
Agar
- Blood Agar Base
- Blood Agar Base No.
2
- Production of High
Quality Blood Agar
- Use of Sodium
Polyanethol Sulfonate in the Preparation of 5% Sheep Blood Agar
Plates
- Conventional Diagnostic
Methods
- Hemolysis - Blood
Agar
- Culture Methods
Applications
- Optochin
Revisited: Defining the Optimal Type of Blood Agar for Presumptive
Identification of Streptococcus pneumoniae
- Isolation and Identification of
Thermophilic campylobacters from diarrhoeal Children in
Baghdad
- Abolition of Swarming
of Proteus by p-Nitrophenyl Glycerin: Application to Blood Agar
Media
- A Mutant of Bacillus
Subtilis with High-Producing Surfactin by Ion Beam Implantation
- Blood Agar and
Mycobacterium tuberculosis: the End of a Dogma
Technology
- New and Emerging
Technology
- An Agar Gel Diffusion
Method for the Identification of Mosquito Blood- Meals
- Pyrosequencing
Bacillus anthracis
- Microbial Cell Culture
Technique and Morphological Analysis Technique
- The agglutinability of
Blood and Agar Strains of Typhoid Bacilli
- Cost-Effectiveness of
Blood Agar for Isolation of Mycobacteria
Standards
- Charcoal Agar
Blood With Cephalexin
- Guidelines for Assuring
Quality of
Medical Microbiological Culture Media
- Inoculation of culture
media
- Quality control of culture media in a microbiology
laboratory
- Quality Control for
Microbiological Culture Media
- Blood and chocolate
agar
Project &
Experts
- Bacteria Growing in Blood
Agar Petri Dishes
- Nile tilapia,
oreochromis niloticus, blood agar and the culture of fish bacterial
pathogens
- Testing Hemolytic
Growth and Nutrient Levels Found Within
- The Effect of
Antiseptics on the Survival Rate of Different Types of Bacteria
- The Effect of
Antibacterial and Non-Antibacterial Hand Soap on the Amount of Bacteria
on the Hands
- The Effect of Mouthwash
on Alpha Streptococcus
- The Effects of
Antibiotics on Bacterial Growth
- Consultant from
USA
- US Consultant from
Alabama
- Consultant from New
York
- US Scientists
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Properties &
Functions
- In vitro Antibacterial
Comparison of Dentifrices Claiming Antigingivitis and Gum-Healing
Properties
- Detection of
Pneumococci in Respiratory Secretions: Clinical Evaluation of Gentamicin
Blood Agar
- A Comparision of
Columbia Blood Agar With or Without Oxolinic Acid for the Isolation
of Streptococci from Throat Swabs
- Isolation of Bacteria
from Ear, Nose and Throat
- Susceptibilities of
Mycobacterium tuberculosis to Isoniazid and Rifampin on Blood
Agar
- As a Bacterial Culture
Medium, Citrated Sheep Blood Agar Is a Practical Alternative to Citrated
Human Blood Agar in Laboratories of Developing Countries
Products
- Azide Blood Agar
Base
- Blood Agra Base,
Improved
- Blood Agra Base w/Low
PH
- Campy Blood Agar
- CDC Anaerobic Blood
Agar
- Columbia Blood
Agar
- Blood Glucose
Agar
- Cystine Tellurite
Blood Agar
- Tryptose Blood
Agar Base
- Blood Agar
- Anaerobe Neomycin 5%
Sheep Blood Agar
- Coagulase
Mannitol Agar
Material Safety
Data Sheet
- Blood Agar Base No.
2
- Columbia CNA Blood Agar
- Tryptose Blood
Agar Base
- Blood Agar
- Sheep Blood Agar
- Blood Agar, Prepared
Microbiological Media
- Blood Agar Base
Patent
- Cell Culture
Medium
- Instant Culture Media
and method of Sterilizing same
- Microbial culture
medium containing agar and iota carrageenan
- Microscope slide having
culture media and method for use
- Novel Enrichments for
Blood Culture Media
- Peptides for use in
Culture Media
Report &
Market
- Comparison of Mannitol
Salt Agar and Blood Agar Plates for Identification and Susceptibility
Testing of Staphylococcus aureus in Specimens from Cystic Fibrosis
Patients
- Evaluation of Crystal
Violet Blood Agar for Primary Isolation and Identification of Group A –
β Haemolytic Streptococcus
- A Study of Shelf-Life
of Critical Culture Media
- Culture Media and
Supplements
- Blood Agar, Chocolate
Agar, and Mycobacterium tuberculosis
- Isolation of
Escherichia Coli from Raw Milk and Milk Products in Relation to Public
Health Sold under Market Conditions at Tandojam
- Hemolytic streptococci
in Raw Market Milk
- Rapid Methods and
Automation in Microbiology: 25 years of
Development and
Predictions
Suppliers
- Selling Leads of
Culture Media
- Suppliers of
Microbiological Media & Sups
- Global Bio Medical
Suppliers
- Blood Agar Plates
suppliers
- Manufacturers and
Suppliers of Blood Agar Plates
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