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  • A hydrogenase is an enzyme that catalyses the reversible oxidation of molecular hydrogen (H2). Hydrogenases play a vital role in anaerobic metabolism.
  • Hydrogen uptake (H2 oxidation) is coupled to the reduction of electron acceptors such as oxygen, nitrate, sulfate, carbon dioxide, and fumarate, whereas proton reduction (H2 evolution) is essential in pyruvate fermentation and in the disposal of excess electrons.
  • Hydrogenase activity of the washed cell suspensions was measured by hydrogen uptake in a standard Warburg apparatus with the bath temperature at 38 C in an atmosphere of hydrogen.
  • The fermentative hyperthermophile Pyrococcus furiosus contains an NADPH-utilizing, heterotetrameric, cytoplasmic hydrogenase that catalyzes both H2 production and the reduction of elemental sulfur to H2S.
  • The hydrogenase, also known as sulfhydrogenase because of its S0-reducing activity, is a Ni-containing, iron-sulfur flavoprotein that also serves to
    reduce H1 to H2. Sulfide dehydrogenase is an iron-sulfur flavoprotein which also functions as a ferredoxin:NADP oxidoreductase.
  • Two distinct hydrogenases, hereafter referred to as "uptake" and "reversible" hydrogenase, were extracted fromAnabaena sp. strain 7120 and parially purified. The properties of the two enzymes were compared in cell-free extracts.
  • Cell-free preparations of the hydrogenase catalyzed an exchange reaction between heavy water and hydrogen.
  • Hydrogenase-containing microorganisms have the ability to produce a constant output of hydrogen gas (H2) from just sunlight and water.
  • Electrons from a carrier combine with hydrogen ions from the cell environment at the hydrogenase's buried H-cluster active site to produce H2. If harnessed properly, hydrogenase and/or hydrogenase-containing organisms could be used to supply affordable and renewable H2 to be used as an energy fuel, and thus solve the "supply" aspect of the future hydrogen economy.
  • Hydrogenase electrodes are capable of consuming hydrogen directly from microbial media, which ensures their use as fuel electrodes in treatment of organic wastes.
  • Adequate uptake hydrogenase activity in soybean bacteroids often increases plant growth, as well as the efficiency of energy utilization during nitrogen fixation.
  • Fe-hydrogenase is a distinct class of hydrogen-producing metalloenzyme, present in a wide variety of prokaryotes and eukaryotes.
  • Rhizobitoxine produced by Bradyrhizobium species strongly prevented derepression of hydrogenase expression in free-living Bradyrhizobium japonicum, although the toxin had no effect on the activity of cells.
  • Fe-hydrogenase functions either in the utilization of hydrogen as a growth substrate or in the disposing of excess electrons by combining them with protons to form hydrogen.
  • The structure of the HndAc domain and the mapping of the HndDN interacting site obtained by heteronuclear NMR spectroscopy, and analyze the role of HndB within this NADP-reducing hydrogenase complex.
  • Sequence similarities between H2ases and complex I (NADH dehydrogenase) were fi rst reported by Böhm et al.and by Pilkington et al. and have been emphasized in several subsequent reports.
General Information
  • Hydrogenase
  • About Hydrogenase
  • Hydrogenases and Nitrogenases

Synthesis & Production

  • A hydrogenosomal [Fe]-hydrogenase from the anaerobic chytrid Neocallimastix sp. L2
  • Nutritional Requirements for Hydrogenase Production by Escherichia coli
  • Hydrogenases from sulphate reducing bacteria and their role in the bioremediation of textile effluent
  • Synthesis of the H-cluster framework of iron-only hydrogenase
  • Nickel-iron hydrogenase


  • Characterization of Hydrogenase II from the Hyperthermophilic Archaeon Pyrococcus furiosus and Assessment of Its Role in Sulfur Reduction
  • Cell-free Hydrogenase from Chlamydomonas
  • Comparative Characterization of Two Distinct Hydrogenases from Anabaena sp. Strain 7120
  • Hydrogenase and Nitrogen Fixation by Azotobacter
  • Gas Transport inside hydrogenase CpI
  • Approaches to developing biological H2-photoproducing organisms and processes
  • Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii
  • The [FeFe] hydrogenase of Nyctotherus ovalis has a chimeric origin


  • Combinatorial Mutagenesis of a Bidirectional Hydrogenase in Chlamydomonas reinhardtii  Dianne Ahmann

  • Hydrogenase electrodes for fuel cells
  • Rhizobitoxine Inhibition of Hydrogenase Synthesis in Free-Living Bradyrhizobium japonicum
  • Fe-Hydrogenase Maturases in the Hydrogenosomes of Trichomonas vaginalis
  • Transcriptional regulation of the uptake [NiFe]hydrogenase genes in Rhodobacter capsulatus
  • Role of Fe-hydrogenase in biological hydrogen production
  • Applications of bacterial hydrogenases in waste
    decontamination, manufacture of novel bionanocatalysts and in sustainable energy


  • Catalytic Center of [NiFe] Hydrogenases. EPR, ENDOR and FTIR Studies
  • Multiple Forms of Bacterial Hydrogenases
  • Hydrogen Technologies - Photobiological Production of Hydrogen
  • Separation of hydrogenase-catalyzed hydrogen-evolution system from electron-donating system by means of enzymic electric cell technique
  • Hydrogen system energy technologies in global, European and Danish perspective
  • Wiring hydrogenases with nanotubes


  • Cell-Free Extracts and Synthesis of active Hydrogenase
  • Methods and Composition for evolving hydrogenase genes
  • Enzymatic Process
  • Heterogenous specific binding assay employing a cycling reactant as label
  • Method for the enzymatic production of hydrogen
  • Hydrogen production using hydrogenase-containing oxygenic photosynthetic organisms
  • Hydrogen-Enhanced Dechlorination In Contaminated Coastal Sediments
  • Artificial Photosynthesis
  • Solar Energy -> BioFuelsMicroalgae / H2/ CO2(Fundamental) ResearchNational, Nordic,EU, International(IEA-H2) Projects
  • Biochemical and Genetic Studies on Hydrogenase and Nitrogenase Enzymes of Rhodobacter species for Improvement of Biohydrogen Production in Photobioreactor.
  • Hydrogenases and Hydrogen Production by Cyanobacteria
  • The Thiocapsa roseopersicina genome project and the use of results in the hydrogenase research
  • Genomic Evolution and the Tree of Life


  • Consultant from Melbourne
  • List of Experts in Hydrogenase
  • Consultant from California
  • Consultant from US
  • US Consultant from Wisconsin

Turnkey Providers

  • Turnkey Providers from India

Function & Properties

  • Iron Hydrogenases and the Evolution of Anaerobic Eukaryotes
  • An Autocatalytic Step in the Reaction Cycle of Hydrogenase from Thiocapsa roseopersicina Can Explain the Special Characteristics of the Enzyme Reaction
  • Characterization of the Oxygen Tolerance of a Hydrogenase Linked to a Carbon Monoxide Oxidation Pathway in Rubrivivax gelatinosus
  • Fe-Only Hydrogenase Structure and Plausible Mechanism
  • Iron-sulfur clusters of hydrogenase I and hydrogenase II of Clostridium pasteurianum
  • The Physical and Catalytic Propertieos f Hydrogenase I1 of Clostridium pasteurianum
  • Purification and Properties of Membrane-Bound Hydrogenase from Azotobacter vinelandii
  • Structure and function of Ni-Fe hydrogenase studied by theoretical methods


  • Metabolic Engineering of Hydrogen Production in Cyanobacterial Heterocysts
  • Hydrogenase and Hydrogen Production 2007: The 8th International Hydrogenase Conference
  • Peptide models for [NiFe] hydrogenase Active site. Search of Protonated [Ni(Cy-S)] and NiFe compounds
  • Solution structure of HndAc: A thioredoxin-like domain involved in the NADP-reducing hydrogenase complex
  • Molecular Biology of Microbial Hydrogenases
  • Hydrogenase of Clostridium acetobutylicum
  • X-Ray Absorption Spectroscopy on the Ni-Fe and Fe-S Cofactors of the Oxygen-Tolerant Hydrogenases from Ralstonia eutropha
  • Hydrogenase
  • Investigation of metalloproteins like hydrogenase and wateroxidase using an array of different physical methods.
  • Maturation of hydrogenase enzymes in Thiocapsa roseopersicina
  • Hydrogen photoproduction: electrochemical and spectroscopic characterization of an algal iron hydrogenase
  • Properties of hydrogenase from Megasphaera elsdenii
  • The Thiocapsa roseopersicina genome project and the use of results in the hydrogenase research

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