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. Amines . Chemicals . Ordering Information. Contact

Project at a Glance Contents on the CD ROM
  • Ninhydrin (2,2-Dihydroxyindane-1,3-dione) is a chemical used to detect ammonia or primary and secondary amines. When reacting with these free amines, a deep blue or purple color known as Ruhemann's purple is produced.
  •  It is a white solid which is soluble in ethanol and acetone at room temperature. [2] Ninhydrin can be considered as the hydrate of indane-1,2,3-trione.
  • Ninhydrin is prepared by oxidation of bis indane 1,3-dione with an oxidising agent in the presence of a catalyst.
  • Ninhydrin is most commonly used to detect fingerprints, as the terminal amines of lysine residues in peptides and proteins sloughed off in fingerprints react with ninhydrin.
  • When ninhydrin reacts with amino acids, the reaction also releases CO2. The carbon in this CO2 originates from the carboxyl carbon of the amino acid. This reaction has been used to release the carboxyl carbons of bone collagen from ancient bones for stable isotope analysis in order to help reconstruct the palaeodiet of cave bears.
  • A color-developing solution for use in automatic amino acid anlaysis by liquid chromatography, which comprises ninhydrin, an organic solvent.
  • A ninhydrin solution is commonly used by forensic investigators in the analysis of latent fingerprints on porous surfaces such as paper.
  • It is used for preparation of hydrindantin by reduction of ninhydrin with ascorbic acid.
  • It is widely used in the analytical chemistry as a reagent for detecting various substances by TLC. Spraying the TLC patterns with ninhydrin reveals the spot of amino acids, peptides, ephedrine, caffeine, morphine, tauphon, neomycin.
  • Photography of latent prints developed with ninhydrin may be enhanced by using a dark green filter with black and white film may improve the contrast.
  • Ninhydrin has been used to detect the presence of soils associated with decomposing bodies (gravesoils).
  • Ninhydrin
  • Properties
  • Ninhydrin in detection of amino acids

Raw Materials

  • Globulin
  • 2,4-Pentanedione
  • Dimethyl phthalate
  • Ethyl Formate


  • Ninhydrin MSDS
  • Data Sheet of Ninhydrin Hydrate
  • Ninhydrin Material Safety Data Sheet
  • Safety Data Sheet of Ninhydrin
  • Ninhydrin Hydrate Data Sheet
  • Material Safety Data Sheet of Ninhydrin
  • MSDS Of Ninhydrin
  • Ninhydrin Safety Data Sheet
  • Product Information sheet of Ninhydrin reagent solution.


  • Detection of Latent Fingerprints on Thermal Paper and Other Surfaces.
  • Estimation of alpha-amino nitrogen in plasma and urine by the colorimetric ninhydrin reaction.
  • Ninhydrin uses in Fingerprinting.
  • Applications of Ninhydrin.
  • The Ninhydrin Reaction and its Analytical Applications.
  • Ninhydrin and Ionic Liquid Reagent mixture and method for the visualization of Amino acids and Peptides.
  • Colorimetric and Fluorometric Method.
  • Process for Dyeing Keratin Fibres with Ninhydrin compounds comprising one or more fused rings.
  • Method of Automatically analyzing amino acids by liquid chromatography and color-developing solution.
  • Process for Dyeing Keratin Fibers with at least one Ninhydrin Derivative.
  • Reagent impregnated paper strips for use in preparing TLC spray reagents.
  • Method of identifying a thief and stolen articles.
  • Use of at least one Ninhydrin Derivative for Dyeing.

Company Profile & Consultants

  • Company from Ahmedabad.
  • Company from Gujarat.
  • Company from India.
  • Company from Maharashtra.
  • Company from Mumbai.
  • Consultants from New York.


  • China Suppliers.
  • Suppliers from India.
  • Hongkong suppliers.


  • Synthesis and Dyeing performance of azo dyes based on schiff base of ninhydrin and 3-amino phenol.
  • Analytical method for determination of glucosamine release from modified release matrix tablets.
  • Effect of Ninhydrin on biochemical & histopathological changes induced by ethanol in gastric mucosa of rats.
  • Ninhydrin-based forensic investigations.
  • Spectrophotometric determination of Lisinopril in pharmaceuticals.
  • Metabolism of Amino acids in diabetes mellitus.
  • Ninhydrin Reagent for photometric determination of amino acids & related compounds.
  • Reaction of Ninhydrin with Dipeptides.
  • Reagent for chemical development of Latent Fingerprints.
  • A Sensitive assay for ornithine amino transferase in rat brain mitochondria by ninhydrin method.


  • Computational Design of Ninhydrin Analogues.
  • The Colorimetric Estimation of Alpha-Amino nitrogen in tissue fluids.
  • The use of DFO for the fluorescent detection of latent fingerprints on Paper.
  • Using Ninhydrin to reconstruct a disturbed outdoor death scene.
  • The sensitivity of approved ninhydrin and biuret tests in assessment of protein contamination on surgical steel.
  • Determination of pipecolic acid after precolumn ninhydrin derivatization using domestic microwave.




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