Bacillus Thuringeinsis
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  • Bacillus thuringiensis  is a Gram-positive, soil-dwelling bacterium, commonly used as a biological alternative to a pesticide; alternatively, the Cry toxin may be extracted and used as a pesticide.
  • B. thuringiensis was first discovered in 1902 by Japanese biologist Shigetane Ishiwatari. In 1911, B. thuringiensis was rediscovered in Germany by
    Ernst Berliner, who isolated it as the cause of a disease called Schlaffsucht in flour moth caterpillars.
  • Bacillus thuringiensis  is a naturally-occurring soil bacterium that produces poisons which cause disease in insects.
  • Bt toxins are considered environmentally friendly by many farmers and may be a potential alternative to broad spectrum insecticides.Large-scale applications of B.t. can have far reaching ecological impacts.
  • Bacillus thuringiensis products available in the United States are comprised of one of five varieties
  • Each of the more than 800 strains of Bacillus thuringiensis may exhibit different toxicity to insects, rodents and humans. An aeration strategy was proposed for foam control in an airlift reactor with double wire mesh draft tubes. The airlift reactor was employed in the cultivation of Bacillus thuringiensis for thuringiensin production.
  • A two-step procedure was used to place a cryIC crystal protein gene from Bacillus thuringiensis subsp. aizawai into the chromosomes of two B. thuringiensis subsp. kurstaki strains containing multiple crystal protein genes.
  • The production of the bioinsecticide can be done using the aerobic cultivation.
  • The preparations of spores and crystals of Bacillus thuringiensis have been used for about 60 years for the control of insects harmful to the cultivated plants and forests and, also, for the control of insect vectors of diseases.
  • The local production of Bt biopesticides using strains isolated in the Laboratory and showing originalities, for the development of local biopesticides  industries and biofarming promotion.
  • Detailed techno-economic analysis of alternative growth substrates, namely, raw wastewater sludge; hydrolyzed wastewater sludge; starch industry wastewater for Bacillus thuringiensis var. kurstaki HD-1 (Bt) biopesticides production in comparison with semi-synthetic commercial soyameal medium was carried out.
  • To develop a cost-effective process for the production of Bacillus thuringiensis based insecticide, it is important to cultivate the bacterial strain in rich medium to obtain the highest yields of spore-crystal complexes.
  • We optimized the PCR method to detect genetically engineered Bacillus thuringiensis (Bt) maize in open quarantine fields in Kenya.
  • In the Effects of Protoplast Fusion on δ-endotoxin Production in Bacillus thuringiensis Spp. (H14) mutant forms of Bacillus thuringiensis spp. israelensis (H14) were produced.
  • Three Bacillus thuringiensis isolates designated 1M, K10-2 and V24-M with varying levels of toxicity to Chilo partellus (spotted stalk borer) were characterized to establish the basis for the differential toxicity and to identify any unique properties that may be used to screen other isolates.
  • Transgenic technology, involving a wide range of pesticidal genes from the bacterium Bacillus thuringinesis (Bt), dominates the scenario of agricultural biotechnology.   At the same time, Bt technology is also the most focused target of vehement anti-tech activism.  
  • The development of the production and use of Bacillus thuringiensis in Brazil at a commercial scale faces
    certain difficulties, among them the establishment of efficient methodologies for the quantitation of toxic products to be commercialized.
  • The recently sequenced 218 kb genome of morphologically atypical Bacillus thuringiensis phage 0305φ8-36 exhibited only limited detectable homology to known bacteriophages. The only known relative of this phage is a string of phage-like genes called BtI1 in the chromosome of B. thuringiensis israelensis.
General Information
  • Bacillus Thuringeinsis
  • Bacillus Thuringeinsis Fact sheet
  • Journal of Bacillus Thuringeinsis
  • Pesticide Information Profiles
  • Bacillus Thuringeinsis Information

Research Institute

  • Research Institute in California
  • Research Institute in China
  • Research Institute in Colorado
  • Research Institute in India
  • Research Institute in Navarra
  • Research Institute in USA

Consultants

  • Consultant from Canada
  • Consultant from India
  • Canadian Consultant
  • Consultant from USA
  • International Consultant

Production

  • Bioinsecticide production by the bacterium Bacillus Thuringiensis
  • Production of Bacillus Thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrients sources.
  • Human Cell Exposure Assays of Bacillus thuringiensis Commercial Insecticides: Production of Bacillus cereus-Like Cytolytic Effects from Outgrowth of  Spores
  • Study and Production of Bacillus Thuringiensis biopesticide.
  • Techno-economic Analysis of Bacillus thuringiensis Biopesticides Production from Wastewater and Wastewater Sludge

Process

  • Activation Process of Dipteran-Specific Insecticidal Protein Produced by Bacillus thuringiensis subsp. israelensis
  • Cultivation of Bacillus thuringiensis in an airlift reactor with wire mesh draft tubes
  • Enhanced Production of Insecticidal Proteins in Bacillus thuringiensis Strains Carrying an Additional Crystal Protein Gene in Their Chromosomes
  • Evaluationof bacterial isolates from salty soils and Bacillus Thuringeinsis strains for the biocontrol of fusarium dry root of potato tubers
  • Improvement of Bacillus thuringiensis Bioinsecticide Production by Fed-batch Culture on Low Cost Effective Medium
  • Production of Bio-inseticide Bacillus thuringiensis var.israelensis in Semicontinuous Processes Combined with Batch Processes for Sporulation
  • Revision of the Nomenclature for the Bacillus thuringiensis Pesticidal Crystal Proteins
  • Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize

Applications

  • The Biopesticide Bacillus thuringiensis and its Applications in Developing Countries
  • Effectiveness of Dead-Spore Bacillus thuringiensis Formulation Against Diamondback Moth
  • Evaluation of Bacillus Thuringiensis isolates against root-knot nematodes following seed application in okra and mungbean
  • Laboratory Assessment of the Effects of Bacillus thuringiensis on Native Lepidoptera

Functions

  • Biological Activity of Bacillus thuringiensis (Berliner) Strains on Larvae and Adults of Ceratitis capitata (Wiedemann) (Diptera:
    Tephritidae)
  • Bacillus thuringiensis crystal protein (δ-endotoxin) gene expression is independent of early sporulation specific functions
  • Genetic modification of Bacillus Thuringiensis Var.Kurstaki HD-73 to overproduce Melanin, UV resistance and their insecticidal potentiality against potato tuber moth.
  • Spore and crystal formation in Bacillus thuringiensis var.thuringiensis during growth in cystine and cysteine.
  • Subspecies-Dependent Regulation of Bacillus thuringiensis Protoxin Genes

Reports

  • American Academy of Microbiology report
  • Bacillus thuringiensis subsp. konkukian (Serotype H34) Superinfection: Case Report and Experimental Evidence of Pathogenicity in Immunosuppressed Mice
  • Microbial Pest Control Agent of Bacillus thuringiensis Report
  • Environmental and health impacts of
    Bacillus thuringiensis israelensis
  • History of Bacillus thuringiensis Berliner research and development
  • Lack of cross-reactivity between the Bacillus thuringiensis derived protein Cry1F in maize grain and dust mite Der p7 protein with human sera positive for Der p7-IgE
  • Comparative genomics of Bacillus thuringiensis phage 0305φ8-36: defining patterns of descent in a novel ancient phage lineage
Technology
  • Biopesticide Production Technology
  • Transgenic BT Technology
  • Methedology for fast evaluation of Bacillus Thuringiensis crystal protien content.
  • Detection of Bacillus thuringiensis genes in transgenic maize by the PCR method and FTA paper technology.
  • Effects of Protoplast Fusion on δ-endotoxin Production in Bacillus thuringiensis Spp.
  • Molecular characterization of Bacillus thuringiensis strains with differential toxicity to the spotted stalk borer, Chilo partellus
  • A real-time PCR method to quantify spores carrying the Bacillus thuringiensis var. israelensis cry4Aa and cry4Ba genes in soil

Project

  • Pilot Project of Bacillus Thuringiensis against western spruce budworm in central Montana
  • Control of Mosquito infestation in the bootheel area of southeast missouri.
  • To determine the potential of Bacillus thuringiensis, for the control of a range of insect pests of stored products in the tropics
  • Development and Application of Microbial Insecticides for Biological Control of Mosquito in Gaza Strip
  • Mesoscale Modeling of the Bacillus thuringiensis Sporulation Network Based on Stochastic Kinetics and Its Application for in Silico Scale-down
  • Bt (Bacillus thuringiensis) Potato Project in South Africa
  • Toxins of Bacillus thuringiensis in Transgenic Organisms: Persistence and Ecological Effects

Patent

  • Bacillus Thuringiensis containing composition
  • Bacillus Thuringiensis sporulation gene
  • Insecticidal composition of Bacillus Thuringiensis admixed with pyrethrum
  • Liquid compositions of Bacillus Thuringiensis
  • Bacillus Thuringiensis cry gene and protein
  • Bacillus Thuringiensis toxin enhancer

Material Safety Data Sheet

  • Agree WG
  • BaritoneTM Bio-Insecticide
  • Costar
  • Deliver
  • Bacillus thuringiensis subspecies israelensis
  • Bacillus thuringiensis
  • Vectobac

Regulatory Facts

  • Re-evaluation Decision Bacillus thuringiensis
  • Environmental Health Criteria of Bacillus thuringiensis
  • EPA's Regulation of Bacillus thuringiensis (Bt) Crops
  • Reregistration Eligibility Decision (RED) Facts of Bacillus thuringiensis
  • Reregistration Eligibility Decision (RED) of Bacillus thuringiensis
  • Review report for the active substance Bacillus thuringiensis ssp. aizawai, strain GC-91

Toxicity

  • Absence of toxicity of Bacillus thuringiensis pollen to black swallowtails under field conditions
  • Toxicity of Bacillus thuringiensis CrylAb toxin to the predator chrysoperla carnea
  • Comparative Toxicity of Bacillus thuringiensis var. israelensis Crystal Proteins in aiuo and in tritvo
  • Toxicity of Bacillus thuringiensis b-exotoxin to Three Species of Fruit Flies (Diptera: Tephritidae)
  • Toxicity of local Bacillus Thuringiensis isolates against drosophila melanogaster
  • Toxicity and Mode of Action of Bacillus thuringiensis Cry Proteins in the Mediterranean Corn Borer, Sesamia nonagrioides (Lefebvre)
  • Toxicity of Bacillus thuringiensis Spore and Crystal Protein to Resistant Diamondback Moth (Plutella xylostella)
  • Toxicity of Bacillus thuringiensis israelensis on the Nontarget Organisms Triops cancriformis, Branchipus schaefferi, Leptestheria dahalacensis (Crustacea: Branchiopoda: Notostraca, Anostraca, Spinicaudata)
  • Toxicity of several -endotoxins of Bacillus thuringiensis against Helicoverpa armigera (Lepidoptera: Noctuidae) from Spain

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