- A hydrogenase is an
enzyme that catalyses the reversible oxidation of molecular hydrogen
(H2). Hydrogenases play a vital role in anaerobic metabolism.
- Hydrogen uptake (H2
oxidation) is coupled to the reduction of electron acceptors such as
oxygen, nitrate, sulfate, carbon dioxide, and fumarate, whereas proton
reduction (H2 evolution) is essential in pyruvate fermentation and in
the disposal of excess electrons.
- Hydrogenase activity of
the washed cell suspensions was measured by hydrogen uptake in a
standard Warburg apparatus with the bath temperature at 38 C in an
atmosphere of hydrogen.
- The fermentative
hyperthermophile Pyrococcus furiosus contains an NADPH-utilizing,
heterotetrameric, cytoplasmic hydrogenase that catalyzes both H2
production and the reduction of elemental sulfur to H2S.
- The hydrogenase, also
known as sulfhydrogenase because of its S0-reducing activity, is a
Ni-containing, iron-sulfur flavoprotein that also serves to
reduce H1
to H2. Sulfide dehydrogenase is an iron-sulfur flavoprotein which also
functions as a ferredoxin:NADP oxidoreductase.
- Two distinct
hydrogenases, hereafter referred to as "uptake" and "reversible"
hydrogenase, were extracted fromAnabaena sp. strain 7120 and parially
purified. The properties of the two enzymes were compared in cell-free
extracts.
- Cell-free preparations
of the hydrogenase catalyzed an exchange reaction between heavy water
and hydrogen.
- Hydrogenase-containing
microorganisms have the ability to produce a constant output of hydrogen
gas (H2) from just sunlight and water.
- Electrons from a
carrier combine with hydrogen ions from the cell environment at the
hydrogenase's buried H-cluster active site to produce H2. If harnessed
properly, hydrogenase and/or hydrogenase-containing organisms could be
used to supply affordable and renewable H2 to be used as an energy fuel,
and thus solve the "supply" aspect of the future hydrogen
economy.
- Hydrogenase electrodes
are capable of consuming hydrogen directly from microbial media, which
ensures their use as fuel electrodes in treatment of organic
wastes.
- Adequate uptake
hydrogenase activity in soybean bacteroids often increases plant growth,
as well as the efficiency of energy utilization during nitrogen
fixation.
- Fe-hydrogenase is a
distinct class of hydrogen-producing metalloenzyme, present in a wide
variety of prokaryotes and eukaryotes.
- Rhizobitoxine produced
by Bradyrhizobium species strongly prevented derepression of hydrogenase
expression in free-living Bradyrhizobium japonicum, although the toxin
had no effect on the activity of cells.
- Fe-hydrogenase
functions either in the utilization of hydrogen as a growth substrate or
in the disposing of excess electrons by combining them with protons to
form hydrogen.
- The structure of the
HndAc domain and the mapping of the HndDN interacting site obtained by
heteronuclear NMR spectroscopy, and analyze the role of HndB within this
NADP-reducing hydrogenase complex.
- Sequence similarities
between H2ases and complex I (NADH dehydrogenase) were fi rst reported
by Böhm et al.and by Pilkington et al. and have been emphasized in
several subsequent reports.
|
General
Information
- Hydrogenase
- About
Hydrogenase
- Hydrogenases and
Nitrogenases
Synthesis &
Production
- A hydrogenosomal
[Fe]-hydrogenase from the anaerobic chytrid Neocallimastix sp. L2
- Nutritional
Requirements for Hydrogenase Production by Escherichia coli
- Hydrogenases from
sulphate reducing bacteria and their role in the bioremediation of
textile effluent
- Synthesis of the
H-cluster framework of iron-only hydrogenase
- Nickel-iron
hydrogenase
Process
- Characterization of
Hydrogenase II from the Hyperthermophilic Archaeon Pyrococcus furiosus
and Assessment of Its Role in Sulfur Reduction
- Cell-free
Hydrogenase from Chlamydomonas
- Comparative
Characterization of Two Distinct Hydrogenases from Anabaena sp. Strain
7120
- Hydrogenase and
Nitrogen Fixation by Azotobacter
- Gas Transport
inside hydrogenase CpI
- Approaches to
developing biological H2-photoproducing organisms and processes
- Identification of
genes required for hydrogenase activity in Chlamydomonas
reinhardtii
- The [FeFe] hydrogenase
of Nyctotherus ovalis has a chimeric origin
Application
Technology
- Catalytic Center of
[NiFe] Hydrogenases. EPR, ENDOR and FTIR Studies
- Multiple Forms of
Bacterial Hydrogenases
- Hydrogen Technologies -
Photobiological Production of Hydrogen
- Separation of
hydrogenase-catalyzed hydrogen-evolution system from electron-donating
system by means of enzymic electric cell technique
- Hydrogen system energy
technologies in global, European and Danish perspective
- Wiring
hydrogenases with nanotubes
Patent
- Cell-Free
Extracts and Synthesis of active Hydrogenase
- Methods and Composition
for evolving hydrogenase genes
- Enzymatic
Process
- Heterogenous
specific binding assay employing a cycling reactant as
label
- Method for the
enzymatic production of hydrogen
- Hydrogen production
using hydrogenase-containing oxygenic photosynthetic organisms
|
Project
- Hydrogen-Enhanced
Dechlorination In Contaminated Coastal Sediments
- Artificial
Photosynthesis
- Solar Energy ->
BioFuelsMicroalgae / H2/ CO2(Fundamental) ResearchNational, Nordic,EU,
International(IEA-H2) Projects
- Biochemical and Genetic
Studies on Hydrogenase and Nitrogenase Enzymes of Rhodobacter species
for Improvement of Biohydrogen Production in Photobioreactor.
- Hydrogenases and
Hydrogen Production by Cyanobacteria
- The Thiocapsa
roseopersicina genome project and the use of results in the hydrogenase
research
- Genomic Evolution and the
Tree of Life
Consultants
- Consultant from
Melbourne
- List of
Experts in
Hydrogenase
- Consultant from
California
- Consultant from
US
- US Consultant from
Wisconsin
Turnkey
Providers
- Turnkey Providers from
India
Function &
Properties
- Iron Hydrogenases and
the Evolution of Anaerobic Eukaryotes
- An Autocatalytic Step
in the Reaction Cycle of Hydrogenase from Thiocapsa roseopersicina Can
Explain the Special Characteristics of the Enzyme Reaction
- Characterization
of the Oxygen Tolerance of a Hydrogenase Linked to a Carbon Monoxide
Oxidation Pathway in Rubrivivax gelatinosus
- Fe-Only Hydrogenase
Structure and Plausible Mechanism
- Iron-sulfur
clusters of hydrogenase I and hydrogenase II of Clostridium
pasteurianum
- The Physical and
Catalytic Propertieos f Hydrogenase I1 of Clostridium
pasteurianum
- Purification and
Properties of Membrane-Bound Hydrogenase from Azotobacter
vinelandii
- Structure and function
of Ni-Fe hydrogenase studied by theoretical methods
Report
- Metabolic
Engineering of Hydrogen Production in Cyanobacterial
Heterocysts
- Hydrogenase and
Hydrogen Production 2007: The 8th International Hydrogenase
Conference
- Peptide models for
[NiFe] hydrogenase Active site. Search of Protonated [Ni(Cy-S)] and NiFe
compounds
- Solution structure of
HndAc: A thioredoxin-like domain involved in the NADP-reducing
hydrogenase complex
- Molecular Biology
of Microbial Hydrogenases
Research
- Hydrogenase of
Clostridium acetobutylicum
- X-Ray Absorption
Spectroscopy on the Ni-Fe and Fe-S Cofactors of the Oxygen-Tolerant
Hydrogenases from Ralstonia eutropha
- Hydrogenase
- Investigation
of metalloproteins like hydrogenase and wateroxidase using an array of
different physical methods.
- Maturation of
hydrogenase enzymes in Thiocapsa roseopersicina
- Hydrogen
photoproduction: electrochemical and spectroscopic characterization of
an algal iron hydrogenase
- Properties of hydrogenase from Megasphaera
elsdenii
- The Thiocapsa roseopersicina genome project and the use of
results in the hydrogenase research
Order the CD-ROM
Today |