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- Luminol (C8H7N3O2) is a versatile chemical
that exhibits chemiluminescence, with a striking blue glow, when
mixed with an appropriate oxidizing agent.
- It is a white to slightly yellow
crystalline solid that is soluble in most polar organic solvents,
but insoluble in water.
- Luminol is used by forensic investigators
to detect trace amounts of blood left at crime scenes as it reacts
with iron found in hemoglobin.
- It is used by biologists in cellular
assays for the detection of copper, iron, and cyanides, in addition
to the detection of specific proteins by Western Blot.
- For analysis of an area, luminol can be
sprayed evenly across the area, and trace amounts of an activating
oxidant will cause the luminol to emit a blue glow that can be seen
in a darkened room.
- Luminol may be synthesized by a reverse
phosphorescence 2-step process. It begins from 3-nitrophthalic
acid.[2][3] First, hydrazine (N2H4) is heated with the
3-nitrophthalic acid in a high-boiling solvent such as triethylene
glycol.
- A condensation reaction occurs, with loss
of water, forming 3-nitrophthalhydrazide. Reduction of the nitro
group to an amino group with sodium dithionite (Na2S2O4) produces
luminol.
- The usage of this chemical is based on the
fact that everything leaves some sort of trace behind. Whenever
blood is spilled, its myriad components can cling to the surface for
years.
- Although the visible stain left behind can
be cleaned, there are several constituents that cannot be removed
without a concentrated effort using heavy duty cleaning agents.
- Some of the most impressive and
aesthetically pleasing chemical reactions known are those that
result in the phenomenon of chemiluminescence.
- While exothermic reactions usually release
energy in the form of heat, some produce little or no heat and
release their energy by the emission of light.
- These "glowing" reactions are generally
oxidations, and a good example is the oxidation of
5-aminophthalhydrazide, or luminol, which produces a brilliant
blue-green light.
- Luminol is used by crime scene
investigators to locate traces of blood, even if it has been cleaned
or removed.
- The investigator prepares a solution of
luminol and the activator and sprays it throughout the area under
investigation. The iron present in any blood in the area catalyzes
the chemical reaction that leads to the luminescence revealing the
location of the blood.
- Luminol chemiluminescence can also be
triggered by a number of substances such as copper or
copper-containing alloys, and certain bleaches and, as a result, if
a crime scene is thoroughly cleaned with a bleach solution or
horseradish, residual cleaner will cause the entire crime scene to
produce the typical blue glow, effectively camouflaging any organic
evidence, such as blood.
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General
- Luminol
- DNA and Luminol
- Luminol - A
chemical to reveal hidden crime scenes
- Luminol - An
illustrating experience
- Luminol, a
chemiluminescent substance
- Luminol Video
MSDS
- 3
Aminophthalhydrazide 98%
- Luminol MSDS1
- Luminol MSDS2
- Luminol
- Luminol datasheet
- Sodium Luminol
- Western blotting
Luminol Reagent
- Luminol Reagent
- 3
aminophthalhydrazide
- Luminol TR
- Luminol Bi
- Luminol Flinn
- Luminol Carolina
Products
- Luminol Sodium salt
- Western blotting
Luminol reagent kit
- Bluestar forensic
- Western blotting
Luminol reagent
- Luminol, Powdered
- Luminol TRi
Synthesis
- Preparation of
Luminol
- Lab Preparation of
Luminol
- Synthesis of
Luminol
- The Synthesis Of
Luminol And A Test Of It’s Chemiluminescent Properties.
- A synthesis of
Luminol
- The synthesis of
Luminol
Raw material suppliers
- 3-nitrophthalic
Acid
- Hydrazine suppliers
- Triethylene Glycol
- Sodium Dithionate
Patent
- Increasing plant
growth with luminol
- Method for
improving chemiluminescent signal
- Shuttlecock with
luminescent means
- Chemiluminescent
analytical method
- Composition and
method for enhancing detection of superoxide anion
- Method for
enhancing detection of superoxide anion
- Luminescent
substrate preparation and its use in specific binding assays
- Composition, kit
and method of detecting and locating traces of blood
- Apparatus for
detection of certain nitrogen-containing gases using
chemiluminescence
- Luminol tagged
polymers for treatment of industrial systems
- Method and
apparatus for eliminating luminol interference material
- Compositions and
methods for utilizing mixed substrate solutions of luminols and
dioxetanes
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Suppliers
- Suppliers of
Luminol
- Manufacturers of
Luminol
- Selling leads of
Luminol
- Exporters of
Luminol
- Luminol Suppliers list
Company profiles
- Company from
Belgaum
- Company1 from China
- Company2 from China
- Company from
Massachusetts
- Company from U.S.A
Consultancy
- Consultancy from
Kansas
- Consultancy from
New York
- Consultancy from
Texas
- Consultancy from
U.S.A
Experiment
- Chemiluminescence
with Luminol
- Oxidation of
Luminol
- Test for blood
- The glowing
reaction
- Luminol experiment
Report
- Luminol test
- A new
high-performance reagent and procedure for latent bloodstain
detection based on Luminol chemiluminescence
- Luminol-enhanced
chemiluminescence induced in peripheral blood-derived human
phagocytes
- Intra- and
extracellular events in luminol-dependent chemiluminescence of
polymorphonuclear leukocytes.
- Inhibition of
Bleach-Induced Luminol Chemiluminescence
- Luminol/HO
chemiluminescence detector for the analysis of nitric oxide
- Fast gas
chromatography with luminol chemiluminescence detection for the
simultaneous determination of nitrogen dioxide and peroxyacetyl
nitrate in the atmosphere
- Luminol and the
crime scene
- Blood-Enhancement
Reagents, Luminol, Bluestar®, Fluorescein, and Hemascein
- Attempted cleaning
of bloodstains and its effect on the forensic luminol test
Study
- Quality Control of
Reactive Oxygen Species Measurement by Luminol
- Phenols as
Enhancers of the Chemiluminescent Horseradish
Peroxidase-Luminol-Hydrogen Peroxide Reaction
- Peroxynitrite-induced
luminol chemiluminescence
- An Investigation of
the Mechanism of the Luminescent Peroxidation of Luminol by Stopped
Flow Techniques
- Increasing the
specificity of the forensic luminol test
- Enzymatic
Activation and Trapping of Luminol-Substituted Peptides and
Proteins.
- The Effect of
Luminol on Presumptive Tests and DNA Analysis Using the Polymerase
Chain Reaction
- Luminol vs.
BlueStar®: A Comparison Study of Latent Blood Reagents
- A
Photographic Comparison of Luminol, Fluorescein, and Bluestar
-
Chemiluminescence of Luminol Catalyzed by Electrochemically Oxidized
Ferrocenes
- Synthesis and
Characterization of Luminol
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