Luminol
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Project at a Glance Contents on the CD ROM
  • Luminol (C8H7N3O2) is a versatile chemical that exhibits chemiluminescence, with a striking blue glow, when mixed with an appropriate oxidizing agent.
  • It is a white to slightly yellow crystalline solid that is soluble in most polar organic solvents, but insoluble in water.
  • Luminol is used by forensic investigators to detect trace amounts of blood left at crime scenes as it reacts with iron found in hemoglobin.
  • It is used by biologists in cellular assays for the detection of copper, iron, and cyanides, in addition to the detection of specific proteins by Western Blot.
  • For analysis of an area, luminol can be sprayed evenly across the area, and trace amounts of an activating oxidant will cause the luminol to emit a blue glow that can be seen in a darkened room.
  • Luminol may be synthesized by a reverse phosphorescence 2-step process. It begins from 3-nitrophthalic acid.[2][3] First, hydrazine (N2H4) is heated with the 3-nitrophthalic acid in a high-boiling solvent such as triethylene glycol.
  • A condensation reaction occurs, with loss of water, forming 3-nitrophthalhydrazide. Reduction of the nitro group to an amino group with sodium dithionite (Na2S2O4) produces luminol.
  • The usage of this chemical is based on the fact that everything leaves some sort of trace behind. Whenever blood is spilled, its myriad components can cling to the surface for years.
  • Although the visible stain left behind can be cleaned, there are several constituents that cannot be removed without a concentrated effort using heavy duty cleaning agents.
  • Some of the most impressive and aesthetically pleasing chemical reactions known are those that result in the phenomenon of chemiluminescence.
  • While exothermic reactions usually release energy in the form of heat, some produce little or no heat and release their energy by the emission of light.
  • These "glowing" reactions are generally oxidations, and a good example is the oxidation of 5-aminophthalhydrazide, or luminol, which produces a brilliant blue-green light.
  • Luminol is used by crime scene investigators to locate traces of blood, even if it has been cleaned or removed.
  • The investigator prepares a solution of luminol and the activator and sprays it throughout the area under investigation. The iron present in any blood in the area catalyzes the chemical reaction that leads to the luminescence revealing the location of the blood.
  • Luminol chemiluminescence can also be triggered by a number of substances such as copper or copper-containing alloys, and certain bleaches and, as a result, if a crime scene is thoroughly cleaned with a bleach solution or horseradish, residual cleaner will cause the entire crime scene to produce the typical blue glow, effectively camouflaging any organic evidence, such as blood.
General
  • Luminol
  • DNA and Luminol
  • Luminol - A chemical to reveal hidden crime scenes
  • Luminol - An illustrating experience
  • Luminol, a chemiluminescent substance
  • Luminol Video

MSDS

  • 3 Aminophthalhydrazide 98%
  • Luminol MSDS1
  • Luminol MSDS2
  • Luminol
  • Luminol datasheet
  • Sodium Luminol
  • Western blotting Luminol Reagent
  • Luminol Reagent
  • 3 aminophthalhydrazide
  • Luminol TR
  • Luminol Bi
  • Luminol Flinn
  • Luminol Carolina

Products

  • Luminol Sodium salt
  • Western blotting Luminol reagent kit
  • Bluestar forensic
  • Western blotting Luminol reagent
  • Luminol, Powdered
  • Luminol TRi

Synthesis

  • Preparation of Luminol
  • Lab Preparation of Luminol
  • Synthesis of Luminol
  • The Synthesis Of Luminol And A Test Of It’s Chemiluminescent Properties.
  • A synthesis of Luminol
  • The synthesis of Luminol
Raw material suppliers
  • 3-nitrophthalic Acid
  • Hydrazine suppliers
  • Triethylene Glycol
  • Sodium Dithionate

Patent

  • Increasing plant growth with luminol
  • Method for improving chemiluminescent signal
  • Shuttlecock with luminescent means
  • Chemiluminescent analytical method
  • Composition and method for enhancing detection of superoxide anion
  • Method for enhancing detection of superoxide anion
  • Luminescent substrate preparation and its use in specific binding assays
  • Composition, kit and method of detecting and locating traces of blood
  • Apparatus for detection of certain nitrogen-containing gases using chemiluminescence
  • Luminol tagged polymers for treatment of industrial systems
  • Method and apparatus for eliminating luminol interference material
  • Compositions and methods for utilizing mixed substrate solutions of luminols and dioxetanes

 

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Suppliers
  • Suppliers of Luminol
  • Manufacturers of Luminol
  • Selling leads of Luminol
  • Exporters of Luminol
  • Luminol Suppliers list

Company profiles

  • Company from Belgaum
  • Company1 from China
  • Company2 from China
  • Company from Massachusetts
  • Company from U.S.A

Consultancy

  • Consultancy from Kansas
  • Consultancy from New York
  • Consultancy from Texas
  • Consultancy from U.S.A

Experiment

  • Chemiluminescence with Luminol
  • Oxidation of Luminol
  • Test for blood
  • The glowing reaction
  • Luminol experiment

Report

  • Luminol test
  • A new high-performance reagent and procedure for latent bloodstain detection based on Luminol chemiluminescence
  • Luminol-enhanced chemiluminescence induced in peripheral blood-derived human phagocytes
  • Intra- and extracellular events in luminol-dependent chemiluminescence of polymorphonuclear leukocytes.
  • Inhibition of Bleach-Induced Luminol Chemiluminescence
  • Luminol/HO chemiluminescence detector for the analysis of nitric oxide
  • Fast gas chromatography with luminol chemiluminescence detection for the simultaneous determination of nitrogen dioxide and peroxyacetyl
    nitrate in the atmosphere
  • Luminol and the crime scene
  • Blood-Enhancement Reagents, Luminol, Bluestar®, Fluorescein, and Hemascein
  • Attempted cleaning of bloodstains and its effect on the forensic luminol test

Study

  • Quality Control of Reactive Oxygen Species Measurement by Luminol
  • Phenols as Enhancers of the Chemiluminescent Horseradish Peroxidase-Luminol-Hydrogen Peroxide Reaction
  • Peroxynitrite-induced luminol chemiluminescence
  • An Investigation of the Mechanism of the Luminescent Peroxidation of Luminol by Stopped Flow Techniques
  • Increasing the specificity of the forensic luminol test
  • Enzymatic Activation and Trapping of Luminol-Substituted Peptides and Proteins.
  • The Effect of Luminol on Presumptive Tests and DNA Analysis Using the Polymerase Chain Reaction
  • Luminol vs. BlueStar®: A Comparison Study of Latent Blood Reagents
  • A Photographic Comparison of Luminol, Fluorescein, and Bluestar
  • Chemiluminescence of Luminol Catalyzed by Electrochemically Oxidized Ferrocenes
  • Synthesis and Characterization of Luminol

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